Current voltage measurements of electrolyte filled microelectrodes with ohmig and rectifying properties.
نویسنده
چکیده
In Übereinstimmung mit der vorangegangenen, theoretischen Ableitung hängen der Widerstand (R0) und die Stromspannungs [i-E) -Kurven von Mikropipetten, die als Elektroden in der Elektrophysiologie verwendet werden, vom Radius r0[cm] und der Konzentration c[Äq./cm 3] des Elektro lyten ab. Große Werte von r0 c [Äq./cm2] entsprechen Volumenleitung in der Öffnung. /?0vol ^ 1/ r0 c Abb. 3; die i-£-Kurven sind ohmisch Abb. 2. Kleine Werte von r0 c entsprechen DoppelschichtLeitung. Äodl^^Ovol und dl ist unabhängig von c und nur wenig abhängig von r0 Abb. 3; die i-£-Kurven sind gleichrichtend Abbn. 2, 6, 7, 8 und 9. Die oberflächenaktive Substanz T h (N 0 3) 4 setzt in geringer Konzentration die Asymmetrie der i-Zs-Kurve herab Abb. 8 und invertiert die Gleichrichtung vollständig bei hohen Konzentrationen Abb. 9. Die zwei Arten gleichrichtender Mi kropipetten haben einander ergänzende Eigenschaften: Beide Ionensorten können in Zellen injiziert werden: Kationen durch normale Gleichrichtung Verwendung des geeigneten Elektrodentyps kann niederohmigen Elektrode ausgeführt werden.
منابع مشابه
Application of active electrode compensation to perform continuous voltage-clamp recordings with sharp microelectrodes.
OBJECTIVE Electrophysiological recordings of single neurons in brain tissues are very common in neuroscience. Glass microelectrodes filled with an electrolyte are used to impale the cell membrane in order to record the membrane potential or to inject current. Their high resistance induces a high voltage drop when passing current and it is essential to correct the voltage measurements. In partic...
متن کاملTraveling-wave electrokinetic micropumps: velocity, electrical current, and impedance measurements.
An array of microelectrodes covered in an electrolyte and energized by a traveling-wave potential produces net movement of the fluid. Arrays of platinum microelectrodes of two different characteristic sizes have been studied. For both sizes of arrays, at low voltages (<2 V pp) the electrolyte flow is in qualitative agreement with the linear theory of ac electroosmosis. At voltages above a thres...
متن کاملAnalysis and Simulation of ZVS Methods in Full Bridge Converters and Realization of a 3 KW Prototype
One of the difficulties with PWM switching converters is high switching loss and electromagnetic interference due to switching at non-zero voltage and current, which limits the operating frequency. In order to reduce the converter volume and weight (by increasing the frequency) and reducing switching losses, zero voltage and current switching methods are recommended. In this paper, four main ze...
متن کاملAnalysis and Simulation of ZVS Methods in Full Bridge Converters and Realization of a 3 KW Prototype
One of the difficulties with PWM switching converters is high switching loss and electromagnetic interference due to switching at non-zero voltage and current, which limits the operating frequency. In order to reduce the converter volume and weight (by increasing the frequency) and reducing switching losses, zero voltage and current switching methods are recommended. In this paper, four main ze...
متن کاملBlock of inward rectification by intracellular H+ in immature oocytes of the starfish Mediaster aequalis
Intracellular pH was recorded in immature starfish oocytes using pH-sensitive microelectrodes, and inwardly rectifying potassium currents were measured under voltage clamp. When the intracellular pH was lowered using acetate-buffered artificial sea water from the normal value of 7.09 to 5.9, inward rectification was completely blocked. The relationship between inward rectification and internal ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete
دوره 24 2 شماره
صفحات -
تاریخ انتشار 1969